动物造模,高脂血症 z-bio 2022-05-16 647

　　Objective: To observe the dynamic changes of blood lipids and the pathological characteristics of cardiovascular complications in a new type of hyperlipidemia-susceptible WSHc rats induced by high-fat diet, and to provide reference for the application of WSHc rats.

　　METHODS: Twenty 7-8 week old female WSHc rats and 10 Wistar rats were selected for high-fat feeding, and 10 WSHc rats and 10 Wistar rats of the same age and gender were fed with common chow. They were used as normal controls respectively. The dynamic changes of blood lipids were observed at 2, 4, 8, 12 and 16 weeks after high-fat feeding. After 16 weeks of feeding, cardiac function and left heart structure were detected by echocardiography. Aortic disease.

　　Results: Compared with the normal control group, serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-c) were significantly increased in WSHc rats and Wistar rats after high-fat feeding for 2 weeks; The levels of TC and LDL-c in rats were: (6.34±2.12) mmol/L and (2.56±0.94) mmol/L, while the levels of TC and LDL-c in high-fat Wistar rats were only (2.93±0.23) mmol/L, (0.63±0.12) mmol/L; during the high-fat feeding period, TC of WSHc rats was always maintained at a high level. After 16 weeks of feeding, the cardiac ejection fraction of WSHc rats increased, and the left ventricular The aortic intima is thickened, the elastic fibers are disordered, and early atherosclerotic lesions are formed. After the Wistar rats were induced by high-fat diet, the cardiac function was improved. and cardiovascular disease was not obvious.

　　Conclusion: WSHc rats are sensitive to exogenous cholesterol, and prone to hyperlipidemia and cardiovascular disease after high-fat feeding, and the levels of TC and LDL-c are closer to clinical hyperlipidemia. It is more suitable for the experimental study of hyperlipidemia and related cardiovascular complications.