动物造模,基因敲除 z-bio 2022-08-09 349

　　Objective: To explore the optimal breeding method and identification method of vitamin D receptor (VDR) knockout mice, and to provide animal models for further study of VDR function and related diseases.

　　Methods: Aiming at the VDR knockout mice constructed in the early stage of the research group, four different mating methods were used to generate offspring mice, the phenotype and genotype of the offspring were counted, and the VDR gene knockout homozygotes (VDR-/-) were compared. The reproductive ability of heterozygous (VDR+/-) mice was analyzed, and the expression of VDR was analyzed at the mRNA and protein levels.

　　Results: VDR-/- mice and VDR+/ + mice had significant differences in phenotype, mainly in hair loss, small body size and decreased mobility. The genotype distribution of the offspring mice produced by the four breeding methods was basically In line with Mendelian inheritance law; VDR-/- interaction mice have poor reproductive ability and cannot be directly used to expand VDR-/- knockout mouse strains, while VDR+/- interaction can successfully obtain VDR-/- mice , and it was detected that the VDR gene knockout trait can be stably inherited to offspring mice, so this method is the best way to expand the strain.

　　Conclusion: This experiment successfully identified the genotype of the offspring mice and established a VDR gene knockout mouse strain, which laid the foundation for the follow-up study of VDR function.