动物造模 z-bio 2022-05-16 297

　　Objective: The purpose of this study is to investigate whether the animal nasal aerosol exposure device can operate normally and generate aerosol particles with a specific particle size, and to explore the air tightness, distribution uniformity and disinfection of the system by disinfectants during operation. Effect.

　　METHODS: An animal nasal aerosol exposure device was used to generate Escherichia coli (E. coli) aerosol and mouse hepatitis virus (MHV-JHM) aerosol, which were treated with 75% ethanol and 3% hydrogen peroxide, respectively. The system was sterilized, and E.coli and MHV-JHM were collected from exposed cabins, leak-prone places and after disinfection. Microbial culture was performed on the collected E. coli; RNA was extracted from the collected MHV-JHM, and one-step RT-PCR was used for detection. Viral RNA in the sample.

　　Results: The median diameter of E.coli aerosol particles produced by the device was (1.27±0.61) μm. Colonies were formed on the sampling liquid coating plate in the exposure chamber. The number of colonies on the NC membranes placed on different ports of the exposure chamber was the same, which was easy to leak. No colonies grew on the sedimentation plate at the place of exposure, and no colonies grew on the sampling plate after disinfection; the MHV-JHM virus RNA in the sampling solution in the exposure chamber was positive, and the viral RNA in the sampling solution at the leak-prone place was negative, and the sample solution after disinfection Viral RNA is negative

　　Conclusion: The animal nasal aerosol exposure device can operate normally to generate aerosol; during the operation, the aerosols pass through each port of the exposure chamber and distribute evenly, and the air tightness is good; the system can be effectively disinfected by using the corresponding disinfectant. The disinfection effect is complete.