动物造模 z-bio 2022-05-16 445

　　OBJECTIVE: To analyze the polymorphisms in the regulatory region of the PPARγ2 gene promoter in Wuzhishan miniature pigs.

　　METHODS: Five pairs of primers were designed according to the 2200bp regulatory region of the porcine PPARγ2 promoter to detect the polymorphism of 57 Wuzhishan miniature pigs in a closed group. Predictive analysis of RNA secondary structure and CpG islands.

　　RESULTS: Nine single nucleotide polymorphisms (SNPs) loci were screened, which were: -1595T/C, -1534G/A, -1262C/A, -1220C/A, and -1017A/G ､-963A/G, ､-955G/A, ､-866A/G, ､-333G/A. None of the SNPs were located in the promoter region identified by the software, but the -333G/A mutation was located in the core promoter (-656~-23bp) ) region. The transcription factor binding site near the mutated SNP site will be changed, the minimum free energy of the RNA secondary structure will change before and after the mutation, and its structure will also change significantly. No CpG island was found in the target sequence.

　　Conclusion: The screened SNPs in the regulatory region of PPARγ2 promoter in Wuzhishan miniature pigs may have an impact on the expression and regulation of this gene, and lay a foundation for further research on its function.