动物造模 z-bio 2023-03-15 546

　　Objective: To screen and optimize ferret microsatellite DNA primers, and to evaluate and analyze the genetic diversity of ferret populations.

　　Methods: Using 21 ferret microsatellite loci, genomic DNA extraction and PCR amplification were performed on 30 randomly selected blood samples of ferrets. Data processing and analysis of STR scan results were performed with Popgene 1.32 software.

　　Results: All 21 microsatellite markers showed genetic diversity. A total of 49 alleles were detected. The number of observed alleles was 1-4, with an average of 2.3; the number of effective alleles was 1.000-3.750, with an average of 1.6685. ; observed heterozygosity 0-0.733 average 0.3216; expected heterozygosity 0-0.642 average 0.3394; Xianglong index 0-1.177 average 1.0768; polymorphic information content 0-0.6100, average 0.5485.

　　Conclusion: The 21 pairs of ferret microsatellite primers screened in this paper were verified by microsatellite scanning analysis to have stable PCR amplification. Balance expectations.