动物造模 z-bio 2022-05-16 335

　　OBJECTIVE: To determine the basic biological indicators of SPF CBA/CaJ mice, and to lay a foundation for better use of this strain of mice for biomedical research.

　　Methods: 6-week-old and 12-week-old CBA/CaJ female and male mice were selected, the weights of the main organs of mice of different genders were weighed, and the blood physiology and biochemical indexes were detected by automatic blood physiology and biochemical analyzer. Flow cytometry was used to detect the proportion of immune cells in peripheral blood.

　　Results: The weight of main organs showed that compared with male and female mice of the same age (6 weeks or 12 weeks), the body weight, heart, liver and kidney weights of male mice were significantly higher than those of female mice (P<0.01); CBA/CaJ of the same sex The body weight of 12-week-old male mice was significantly higher than that of 6-week-old mice (P=0.000). Hematological physiological indicators showed that MCV, RDW, PLT, P-LCR, BUN and CHOL of 6-week-old male mice were significantly higher than those of female mice (P<0.01), while the MCHC of males was significantly lower than that of females (P=0.000). Blood biochemical indexes showed that ALKP of 12-week-old males was significantly lower than that of females (P=0.001), and CHOL was significantly higher than that of females. (P=0.000). MCV, RDW, MCH and ALKP of CBA/CaJ mice of the same sex at 12 weeks were significantly lower than those at 6 weeks (P<0.01), while BUN was significantly higher than those at 6 weeks (P<0.01). Peripheral The proportion of blood immune cells showed that B cells, T cells, NK cells and granulocytes of this strain of mice all accounted for a certain proportion.

　　Conclusion: The basic biological data of CBA/CaJ mice are established. CBA/CaJ mice are immunocompetent mice, and their age and gender have certain effects on their basic biological indicators.