动物造模 z-bio 2022-05-19 390

　　Objective: To investigate the feasibility of PLCζ as an activator and its effect on oocyte fertilization and embryonic development after ROSI.

　　Methods: The recombinant plasmid pCRII-TOPO-PLCζ was constructed in vitro, and the expression was induced by mass spectrometry identification and Western blot detection of antigenicity. The recombinant PLCζ protein was used as the activator of mouse ROSI oocytes, and the fertilization and embryo development were recorded and analyzed statistically.

　　Results: The recombinant plasmid pCRII-TOPO-PLCζ was successfully constructed. After in vitro expression, it was identified as mouse PLCζ protein by mass spectrometry, and had good antigenicity. Fertilization rate, 2-4 cell rate, oocyte rate after recombinant PLCζ protein was activated 8 There was no significant difference between the cell rate and cyst formation rate and the blank group (P>0.05).

　　Conclusion: The recombinant PLCζ protein in this study has no significant effect on oocyte fertilization and embryonic development, and its feasibility as an oocyte activator after ROSI in mice is questionable.