动物造模 z-bio 2023-03-04 589

　　Objective: To analyze the structure and composition of the fecal flora of wild tree shrew by high-throughput sequencing technology, and to lay a foundation for the further development and utilization of this new experimental animal.

　　Methods: Three adult male tree shrews were captured in the wild in Kunming, Yunnan Province. The feces were collected, and the V3-V4 regions were amplified with bacterial 16S rRNA universal primers. The Illumina MiSeq sequencing platform was used to study tree shrew fecal microorganisms.

　　Results: A total of 181,657 valid sequences and 624 OTUs were obtained by sequencing. The shannon dilution curve proved that the sequencing amount could cover most of the species in the samples. A total of 9 phyla, 17 classes and 31 bacteria were identified in tree shrew feces. There are 56 families, 124 genera and 172 species. Among them, (1) the dominant phyla are Firmicutes and Bacteroidetes, accounting for 68.38% and 20.52%, respectively; (2) abundant The class with the highest degree is Bacilli with 54.81% and Bacterodia with 20.52%; (3) Lactobacillales and Bacteroidales have the highest abundance with 50.01% and 20.52% respectively. %; (4) the dominant bacterial families are Streptococcaceae and Prevotellaceae, accounting for 40.52% and 12.13%; (5) Lactobacillus and Streptococcus The abundance was the highest, 20.03% and 19.62%, respectively; (6) The abundance of beneficial bacteria such as Lactobacillus and Lactococcus was relatively high, and the abundance of Bifidobacterium was low, but all of them were contained in the tested samples; (7) 16s function prediction found that amino acid transport and metabolism, carbohydrate transport and metabolism and other functions have high abundance.

　　Conclusion: Using high-throughput sequencing technology, the fecal flora of wild tree shrew has been comprehensively detected for the first time. The fecal bacterial composition of wild tree shrew is rich in diversity, and many of them have not been classified and identified with high relative abundance. bacteria, further research is required.