动物造模 z-bio 2023-03-04 406

　　OBJECTIVE: To accumulate valuable research data on rat hepatic bile duct hyperplasia for related scientific research and new drug safety evaluation.

　　Methods: The rats were divided into 3 groups. The first group had 30 male and 30 animals (imported SD rats); the second group had 60 males and 60 males (domestic SD rats); the third group had 60 males and 60 males (domestic Wistar rats). At the end of the experiment, all experimental animals were euthanized, systematically dissected, liver sliced, histopathological examination and immunohistochemical study.

　　Results: Rats in each group had different degrees of bile duct hyperplasia in the hepatic portal area, and the total incidence was 32.33%. The incidence of domestic SD rats was significantly higher than that of imported SD rats (26.67%: 1.67%); domestic Wistar rats The incidence of rats was significantly higher than that of domestic SD rats (53.33%: 26.67%); the incidence of male animals was significantly higher than that of female animals (20%: 12.33%). Pathological observation showed a variety of bile duct hyperplasia and fibrosis changes. The incidence of lesions in grade I and II rats was 84.5%, and the incidence of grade III lesions was only 15.5%. The proliferation of oval cells was consistent with the bile duct hyperplasia lesions, and showed differentiation towards the bile duct epithelium.

　　Conclusion: There are differences in the incidence of hepatic bile duct hyperplasia among rats of different strains and genders. The results of this study provide reference materials for the study of hepatic bile duct hyperplasia in animals and humans under ageing conditions.