动物造模 z-bio 2022-05-25 453

　　OBJECTIVE: To use nucleic acid detection technology to detect MHV, Reo-3 and MNV enteroviruses in mouse cecal contents, and to compare the results with serological detection results to analyze the applicability of nucleic acid detection in mouse health monitoring.

　　Methods: The cecal content samples of mice received in our laboratory were randomly selected, nucleic acid was extracted, three enteroviruses MHV, Reo-3 and MNV were detected by fluorescence quantitative PCR technology; and the correlation analysis was performed with the results of serological antibody detection.

　　RESULTS: Among the 272 samples, the detection rates of MHV, Reo-3 and MNV were 17.3%, 18.8%, and 16.9%, respectively; the detection rates of MHV, Reo-3 nucleic acid were mostly low copy state; The correlation analysis of the serological antibody test results showed that the nucleic acid test results of the three viruses were not completely correlated with the antibody test results.

　　CONCLUSIONS: There is a possibility of carrying MHV and Reo-MNV in the experimental animal mouse population. As a supplement to the serological antibody detection technology, nucleic acid detection can be used as a highly sensitive method in the health monitoring of experimental animal populations.