动物造模 z-bio 2022-05-25 717

　　OBJECTIVE: To screen and optimize marmoset microsatellite DNA primers for genetic analysis and evaluation of the experimental marmoset population introduced by the Institute of Medical Laboratory Animals, Chinese Academy of Medical Sciences.

　　METHODS: Using 20 pairs of marmoset microsatellite primers, randomly selected 30 marmoset blood samples were subjected to genomic DNA extraction and PCR amplification. The amplified products were identified by electrophoresis and then detected by STR scanning. STR scan results for data processing and analysis.

　　Results: All 20 pairs of microsatellite primers showed genetic diversity, and a total of 147 alleles were detected. The number of observed alleles ranged from 5 to 10, with an average of 7.35; the number of effective alleles ranged from 2.2500 to 6.3830. The observed heterozygosity is 0.000-0.4667, the average is 0.1533; the expected heterozygosity is 0.424-0.4350, the average is 0.2506; the Xianglong index is 1.242-2.242-2.0324 , with an average of 1.5949; the polymorphic information content ranged from 0.5366 to 0.8254, with an average of 0.7053.

　　Conclusion: The 20 pairs of marmoset microsatellite primers screened in this paper have high genetic diversity and are in Hardy-Weinberg equilibrium.