动物造模 z-bio 2022-11-21 376

　　OBJECTIVE: To investigate the effects of age and gender on the physiological and biochemical parameters of SJ5-SPF chickens.

　　Methods: The blood biochemical indexes of SJ5-SPF chickens of different ages were determined by automatic blood analyzer, including alanine aminotransferase (ALT), alkaline phosphatase (ALP), glutamyl transferase (GGT), aspartate aminotransferase ( AST), lactate dehydrogenase (LDH), total protein (TP), globulin (GLB), albumin (ALB), total bilirubin (TB), direct bilirubin (DB), indirect bilirubin ( IBIL), glucose (GLU), triglyceride (TG), cholesterol (TCH), urea nitrogen (BUN), creatinine (CRE), potassium (K), sodium (Na), calcium (Ca), a total of 19 items. At the same time, the RM6240C multi-channel physiological signal acquisition and processing system was used to measure the body temperature (T), respiratory rate (R), heart rate (HR), diastolic blood pressure (DBP) and systolic blood pressure (SBP) of SJ5-SPF chickens of different ages.

　　Results: (1) Among the five indicators of body temperature, respiratory rate, heart rate, diastolic blood pressure and systolic blood pressure measured by physiological constants, there were significant differences between groups at 4 weeks of age compared with 20, 25, and 40 weeks of age (P < 0.05), there was only no difference in body temperature between males and females at the same week of age; diastolic blood pressure was only significantly different at 40 weeks of age (P < 0.01); There were extremely significant differences between males and females (P < 0.01); while there were significant differences in respiratory rate and systolic blood pressure between females and males at 4 weeks of age (P < 0.05). (2) The measurement of 19 blood biochemical indicators showed that compared with the groups, there was no significant difference in alanine aminotransferase between females of different ages (P > 0.05), and only alanine aminotransferase and glucose in males had no significant differences. (P > 0.05), and other biochemical indicators had certain differences; compared between males and females at the same age, glutamyl transferase, alanine aminotransferase, aspartate aminotransferase, total bilirubin, direct bilirubin, indirect bilirubin and There were significant differences in erythrocytes, glucose, urea nitrogen and sodium only at 1 week of age (P < 0.05), and there were significant differences in lactate dehydrogenase, total protein, globulin, albumin and creatinine at 2 weeks of age. (P < 0.05), alkaline phosphatase, triglyceride and calcium were significantly different at 3 weeks of age (P < 0.05), while cholesterol and potassium were significantly different at 4 weeks of age (P < 0.05). < 0.05).

　　Conclusion: The results of this study can provide a scientific basis for disease diagnosis, animal quarantine and related research in SJ5-SPF chickens.