动物造模 z-bio 2023-03-02 476

　　OBJECTIVE: To investigate a stable, not easy to fall off, and continuous and timed blood collection method for the common carotid artery cannulation of New Zealand rabbits.

　　Methods: After the animals were anesthetized, the left common carotid artery was dissected out. After successful puncture with a BD closed venous indwelling needle (Y-shaped), the mousseline was ligated and fixed, and then the sternomastoid muscle was sutured at the anterior, middle and posterior points of the indwelling needle. It is fixed with the sternohyoid muscle to prevent the cannula from coming out. The catheter is close to the skin and goes around the neck, and is sutured and fixed to the skin on the back of the neck.

　　Results: The blocking rate of the carotid artery duct in the control group was 35%, while that in the modified group was 5%; the carotid artery duct shedding rate in the control group was 30%, and the experimental group did not fall off. Significant (P<0.05).

　　Conclusion: Three-point suture fixation of the BD needle in the left common carotid artery of New Zealand rabbits can limit the front, back, left, right, up and down movement of the BD needle, is stable and effective, has a long indwelling time, and can continuously collect blood, laying a foundation for the success of the experiment.