动物造模 z-bio 2022-06-01 273

　　OBJECTIVE: To detect non-invasive pulmonary function indexes in C57BL/6 mice, and to provide a basis for the monitoring of pulmonary function in experimental studies in mice.

　　Methods: The pulmonary function indexes of 460 C57BL/6 mice were detected by whole-body plethysmography, the results were statistically analyzed, and the reference value range was determined by percentile method.

　　Results: The inspiratory time (Ti) of mice was 64.7 (55.30~82.60) ms, the expiratory time (Te) was 83.4 (71.70~109.20) ms, the ratio of peak expiratory time (Rpef) was 0.21 (0.16~0.28), and the end of inspiration Pause (EIP) 2.19 (1.96-3.76) ms, end-expiratory pause (EEP) 1.67 (0.12-9.15) ms, tidal volume (TVb) 0.44 (0.25-0.58) mL, airway stenosis index (Penh) 1.29 (0.9) ~2.00), apnea (PAU) 1.18 (1.00~1.64), mid-expiratory flow rate (EF50) 0.64 (0.30~0.98) mL/s, relaxation time (Tr) 39.0 (32.40~51.50) ms, maximum inspiratory flow rate (PIF) 9.74 (5.33~12.83) mL/s, maximum expiratory flow rate (PEF) 9.86 (5.12~13.47) mL/s, respiratory rate (f) 412 (331~474) BPM, ventilation per minute (MVb) 174.4(86.69~235.04)mL.

　　Conclusion: The determination of the reference range of non-invasive pulmonary function indexes in C57BL/6 mice can provide an experimental reference for the basic research of respiratory system diseases.