动物造模,呼肠孤病毒 z-bio 2022-06-01 249

　　Objective: To investigate the characteristics of tree shrew reovirus-induced immune changes, and to provide theoretical basis for virus prevention in tree shrew.

　　Methods: 40-50-day-old tree shrews were selected and divided into three groups (MRV1/TS/2011 virus strain, MRV3/TS/2013 virus strain and blank control). , 28 days of tail vein blood samples, RT-PCR to detect viral load, flow cytometry to detect CD4/CD8/CD19, ELISA to detect IFN-γ.

　　RESULTS: On the 14th day of infection, tree shrew viral load, CD4 + and CD19 + cell numbers in MRV1 / TS / 2011 and MRV3 / TS / 2013 groups reached the peak value, among which CD4 + Compared with the control group, the number of CD8 + cells and the expression of IFN-γ reached a peak value on the 21st day after infection. The expression level of CD4+ was higher after infection with MRV1/TS/2011, and the expression level of CD8+ was higher after infection with MRV3/TS/2013.

　　Conclusion: After MRV1 / TS / 2011 and MRV3 / TS / 2013 infect the body, the changes of CD4 / CD8 / CD19 and IFN-γ production with the change of viral load are preliminarily concluded: MRV1 / TS / 2011 is mainly stimulated in the early stage of infection. CD4 + cells play a major role in humoral immunity of the body; MRV3/TS/2013 may mainly affect the cellular immunity of the body, and humoral immunity only plays a role in the significant increase in virus expression and in the later stage of infection. CD8 + cells and IFN-γ participate in cellular immunity through cellular immunity. Antiviral response. CD4+ cells may be more sensitive to type 1 reovirus, while CD19+ cells may be more sensitive to type 3 reovirus. It has certain guiding significance for the research on the infection characteristics of tree shrew reovirus and the virus prevention of tree shrew itself.