动物造模 z-bio 2022-06-01 248

　　Objective: To study the relationship between different stages of brain development and cognitive function in rats.

　　Methods: 1-month-old, 2-month-old and 8-month-old rats were selected to simulate juvenile, young and adult rats for behavioral comparison. Reward operant conditioning and Morris water maze were used to investigate the differences in exploration interest, manipulation and identification abilities, and spatial learning and memory of rats of different months of age.

　　RESULTS: There was no significant difference in cognitive function between 1-month-old and 2-month-old rats in reward operant conditioning and water maze test. In the stage of reward conditioning, compared with 1-month-old rats, the number of nasal touches of 8-month-old rats decreased (P < 0.01), and there was no significant difference in the correct rate of nasal contact; The pedaling times and pedaling accuracy of the rats were significantly decreased (P < 0.05 or P < 0.01), and the pedal latency was prolonged (P < 0.05). The number of pedals, the number of rewards, and the visual recognition index were significantly decreased (P < 0.05 or P < 0.01). In the Morris water maze test, the total run and platform-seeking latency of 8-month-old rats in the spatial learning stage were significantly increased (P ＜ 0.05), and the average speed was also significantly greater than that of 1-month-old rats (P ＜ 0.05 or 0.05). P < 0.01); in the spatial memory stage, the run ratio and time ratio of the 8-month-old rats in the target quadrant were significantly reduced (P < 0.01).

　　Conclusion: There are differences in the cognitive function of rats in different brain development stages. The cognitive ability of juvenile and young rats is similar, while the exploratory interest, executive operation ability, recognition and spatial learning and memory ability of 8-month-old adult rats Cognitive function has declined.