动物造模 z-bio 2022-06-01 292

　　Objective: To establish a safe and effective method for obtaining liver and kidney samples by in vivo puncture of rhesus monkeys under the guidance of B-ultrasound.

　　METHODS: Four rhesus monkeys, male, 8-12 kg, 10-12 years old, were anesthetized by intramuscular injection of 5-10 mg/kg ketamine. The abdominal area below the costal margin was shaved and routinely disinfected with iodophor. The B-ultrasound guide frame was installed on the 3.5Hz color computerized sonography probe, and the intercostal area of the right upper abdomen of the macaque and the lower abdominal area of the side lumbar costal margin were explored to find the liver or kidney to be punctured. The MyLab 30CV B-ultrasound further adjusted the probe. In order to confirm the target, pay attention to avoid the portal vein and inferior vena cava and other large blood vessels, adjust the range of the biopsy gun to 15 mm, insert the puncture needle into the abdomen vertically through the skin about 1 cm under the guidance of the puncture guide frame, and observe the real-time B-ultrasound image. , so that the puncture target and the puncture needle are located in the guide line, that is, the puncture target and the puncture needle are vertical parts, press the trigger button of the puncture gun to puncture, and quickly pull out the puncture needle after puncturing. The obtained liver and kidney tissues were subjected to RNA extraction.

　　RESULTS: The amount of liver or kidney tissue samples obtained by each puncture of each animal was converted to about 13 mg. The extracted RNA was of high purity and integrity. The operation of this method was fast, reliable and safe. All experimental monkeys recovered well after surgery. There was no major bleeding or death.

　　Conclusion: This method is of great significance for establishing the safe and effective acquisition of rhesus monkey liver and kidney tissue samples under the guidance of ultrasound. The method can reduce research costs, improve the quality of life and animal welfare of laboratory non-human primates, and at the same time ensure the accuracy of experimental results.