动物造模 z-bio 2022-06-02 412

　　OBJECTIVE: To optimize an easy-to-operate, fast and practical method to obtain mouse hepatocytes on the basis of traditional hepatocyte extraction methods, and to provide an improved experimental method reference for researchers engaged in liver function.

　　Methods: The mouse livers were reversely perfused with liver separation solution, then cut into pieces, digested with liver enzymes, and then separated by density gradient centrifugation, and transferred to culture medium for culture. Cell viability was calculated by trypan blue staining, purity was calculated by flow cytometry, and cell morphology was observed by inverted microscope.

　　RESULTS: Hepatocytes with high purity and viability were obtained, with typical morphological characteristics of hepatocytes.

　　Conclusion: Through reverse perfusion, the difficulty of perfusion is reduced, and high-purity and high-viability hepatocytes can be obtained at the same time. It has been proved by many experiments that this method is indeed an easy-to-operate, efficient and applicable method for extracting hepatocytes.