动物造模 z-bio 2022-06-07 281

　　Objective: To study the differences in organ index and expression of hypoxia-related genes between Bama pigs and Bama minipigs.

　　Methods: TaqMan probe real-time quantitative PCR (real-time quantitative PCR) technology was used to detect the changes in the expression of hypoxia-responsive genes in the heart, liver, spleen, lung and kidney of Bama pigs and Bama miniature pigs.

　　Result: The kidney and spleen indices of Bama pigs were lower than those of Bama miniature pigs, and the heart and lung indices of Bama miniature pigs were higher than those of Bama miniature pigs. The expression levels of Vascular endothelial growth factor (VEGF) and hypoxia inducible factor-1α (HIF-1α) in the lungs and kidneys of Phenma pigs were significantly higher than those of ordinary pigs (P<0.05). or P<0.01); erythropoietin (EPO) was only higher in lungs than in Bama minipigs (P<0.05).

　　Conclusion: The organ index of different species may be the result of evolutionary adaptation of species to comprehensive factors such as material environment, energy environment, external pathogens, and energy metabolism needs. The hypoxia-related genes of Bama pigs were significantly higher than those of Bama miniature pigs, revealing that Bama pigs have strong plateau adaptability in terms of genes.