动物造模 z-bio 2022-10-22 322

　　Objective: To irradiate the skin tissue of Japanese white rabbit with LED white light source, and to preliminarily evaluate the safety of LED light source irradiation.

　　Methods: Animals were randomly divided into irradiation group and control group. The animals in the irradiation group were irradiated with the back hair, and the LED light source was irradiated with an intensity of 50 mw/cm2. The animals in the control group were irradiated with the ordinary fluorescent light source. Both groups were irradiated for 5 hours a day for 3 consecutive months. Observe whether the animal skin is red and swollen, and measure the changes of the animal's food, body weight, body temperature, etc.; after irradiation, collect peripheral blood to measure routine and biochemical indicators; Elisa analyzes the changes of immune cells and cytokines; pathological detection of organs, and analysis of skin elastic fibers Changes; skin immunohistochemical analysis of the expression changes of C-myc, P53, cyclin D1 (CCND1) and other oncogenes and tumor suppressor genes.

　　Results: There was no redness and swelling in the skin of the irradiated rabbits, the body weight was slightly higher than that of the control group after 6 weeks, the body temperature was different at 3 weeks and 6 weeks, and there was no abnormal eating; , but within the normal range; ELISA analysis of blood immune cells CD3T cells, cytokines IL-6, TNF-α, IFN-γ levels showed no significant difference between the two groups, the irradiation group CD19B cells, IL-4 levels increased; There were no obvious abnormal structural changes in the histopathological organs of the animals in the group, and no abnormal distribution of skin elastic fibers; there was no difference in the expressions of cyclin (CCND1), C-Myc and P53 proteins in the skin tissue compared with the control group.

　　Conclusion: The Japanese white rabbits were irradiated with LED light source for 3 months, which did not cause obvious histopathological changes in vivo, nor did it cause changes in skin tumor-related factors.