动物造模 z-bio 2022-06-08 252

　　Objective: Using the co-culture method of bovine embryos and endometrial epithelial cells in vitro, to explore the changes of integrin αv, β3 gene differential expression in endometrial epithelial cells before and after co-culture, in order to establish a fast and effective bovine uterine epithelial cell in the future. The subject prediction method provides a reference.

　　Methods: The bovine blastocysts flushed out in vivo were co-cultured with bovine endometrial epithelial cells that were primary cultured and passaged and purified to the fifth passage for 24 h in vitro. Epithelial cells were tested for the expression of αv, β3 genes.

　　Results: RT-PCR detection results showed that integrin αv and β3 were expressed before and after co-culture. In co-culture group I (blastocyst group), the expression of integrin αv and β3 genes induced after co-culture was different from that without co-culture. There was no significant difference between the two in the culture control group (P > 0.05); the expression of integrin αv and β3 genes in the co-culture group Ⅱ (hatching blastocyst group) after co-culture was significantly higher than that in the control group and the co-culture group I (P < 0.05).

　　Conclusion: In vitro co-culture of bovine hatched blastocysts induces bovine endometrial epithelial cells to express integrin αv, β3, which is significantly better than that of early blastocysts; integrin αv, β3 gene can be used as a marker for in vitro detection of bovine uterine receptivity potential.