动物造模 z-bio 2022-06-08 244

　　Objective: To obtain the data of the phenotype and proportion of NK cells in the peripheral blood lymphocytes of Bama pigs, and to establish a high-efficiency method for inducing and culturing porcine CIK cells in vitro.

　　Methods: The peripheral blood lymphocytes of Bama miniature pigs were isolated, and the phenotype of porcine NK cells was characterized by detecting CD2+/CD8+/CD3- cells; by optimizing the induction culture conditions, the ratio of CIK in the induced lymphocytes was increased, and high-efficiency CIK cells were established In vitro induction method.

　　Results: Using the optimized induction culture conditions, the proportion of CD3-/CD2+/CD8+ NK cells on the 5th day of induction culture was as high as 43.63%, which was 5.59 times higher than that of the initial isolation. Three obvious fluorescence peaks were seen on the 5th day, indicating that the cells divided three times after induction, which theoretically increased by 8 times compared with the initial separation. The apparent rise in surface markers is also consistent with the flow analysis results.

　　Conclusion: An efficient method for inducing and culturing porcine CIK cells in vitro has been established.