动物造模,乳腺肿瘤 z-bio 2022-08-19 497

　　Objective To establish a tree shrew mammary tumor model.

　　Methods Using the method of DMBA combined with synthetic progesterone MPA, 45 female tree shrews were selected and randomly divided into 3 groups. (1) DMBA group: DMBA (20 mg/time) was administered by intragastric administration for 3 consecutive times, once a week; (2) DMBA+MPA group: once every 3 weeks, after 3 consecutive intragastric administration of DMBA, at The MPA sustained-release tablet (150 mg/tablet, 90 d sustained-release) was implanted subcutaneously on the left side of the back of tree shrew for the first time, and the MPA tablet was implanted for the second time at an interval of 3 months; (3) Normal control group: using peanut oil Gavage treatment was performed once every 3 weeks for 3 consecutive times. After the experimental treatment, the tumor occurrence was observed every week for a total of 45 weeks. The pathological types of induced tumors were identified by HE staining.

　　Results DMBA alone could induce tree shrew-specific mammary tumors, and the induction rate was 12%; combined with subcutaneous implantation of MPA, the incidence of DMBA-induced mammary tumors could be increased to 50%; while the occurrence of mammary tumors was not observed in the control group. The induced tumors were mainly intraductal papilloma with low malignancy, and only one case was invasive ductal carcinoma with high malignancy.

　　Conclusions The induced intraductal papilloma and invasive ductal carcinoma in tree shrew are both common tumor pathological types in humans. The morphology of the induced tumor was similar to that of the spontaneous tumor.