动物造模 z-bio 2022-06-14 579

　　Objective: To observe the changes of inflammatory response after space-mutated Escherichia coli infection of tail-suspended mice simulated weightlessness.

　　Methods: 40 C57BL/6 mice were randomly divided into control, control infection, tail suspension and tail suspension infection groups. ELISA and RT-qPCR were used to detect the inflammatory factor TNF-α in mouse plasma and intestinal tissue, respectively. , IL-1β, IL-6 content and mRNA expression, HE staining to observe the morphological changes of the small intestine.

　　Results: Plasma inflammatory factor ELISA and intestinal tissue inflammatory factor PCR results showed that compared with the control group, the expressions of inflammatory factors TNF-α, IL-1β and IL-6 in the plasma and intestinal tissue of the mice in the experimental group were increased. , and the tail hanging bacteria group was the most significant (P < 0.01 or P < 0.001); HE staining results of small intestinal tissue showed that the small intestinal mucosa of the experimental group had different degrees of damage, and the tail hanging bacteria group was the most serious.

　　Conclusion: Space-mutated E. coli infection of tail-suspended mice can significantly increase the expression of inflammatory factors in plasma and intestinal tissue, resulting in more severe intestinal mucosal barrier damage, suggesting that space-mutated E. coli infection after tail suspension simulates weightlessness Can cause the body's inflammatory response to increase.