动物造模】,内毒素血症 z-bio 2022-08-10 369

　　Objective: To analyze the changes of miRNA expression profile in myocardial tissue of endotoxin, also known as lipopolysaccharide rats, and to explore the role of miRNA in endotoxemia myocardial injury.

　　Methods: Twenty male SD rats were randomly divided into control group (n=10) and LPS group (n=10). The LPS group was intraperitoneally injected with LPS 10 mg/kg, and the control group was intraperitoneally injected with the same volume of normal saline. After 24 hours, the rats were sacrificed by cervical dislocation, and the myocardial tissue was collected. The expression levels of TLR4, TNF-α and IL-1β were detected by Real-time PCR, and the ultrastructural changes were observed by transmission electron microscope. miRNA chips were used to screen differentially expressed miRNAs in myocardial tissue, and Real-time PCR was used to verify the actual expression levels of candidate miRNAs.

　　RESULTS: The expression of TLR4, TNF-α and IL-1β in myocardial tissue of LPS group was significantly increased, and the ultrastructural changes such as cytoplasmic vacuoles, mitochondrial edema and structural damage occurred in myocardial cells. Microarray technology and Real-time PCR confirmed that miR-194-3p, miR-344a-3p, miR-465-3p, miR-501-5p, miR-3596c, miR-185-3p, miR -877 was significantly up-regulated, and miR-208b-3p, miR-547-3p, miR-141-3p, miR-28-5p, miR-3585-5p were significantly down-regulated.

　　Conclusion: These differentially expressed miRNAs found in the myocardial tissue of endotoxemia rats may be related to the occurrence of myocardial injury.