动物造模 z-bio 2022-06-14 241

　　Objective: To investigate the ideal dose of propofol for intraperitoneal anesthesia in neonatal rats and the distribution of propofol in different brain regions of neonatal rats.

　　Methods: Part 1: The neonatal rats were randomly divided into 5 groups, and different doses of propofol were injected intraperitoneally respectively, and the anesthesia state, maintenance time, and arterial blood gas were recorded respectively, and the ideal dose of propofol for intraperitoneal anesthesia of neonatal rats was obtained by comparison. . Part II: The newborn rats were randomly divided into anesthesia group (group A) and sedation group (group S). According to the experimental results of the first part, sedative and anesthetic doses of propofol were injected intraperitoneally, and the decapitation method was performed when the depth of anesthesia was appropriate. Rats were sacrificed, and the concentration of propofol in different brain regions of rats was determined by high performance liquid chromatography.

　　RESULTS: The intraperitoneal injection of 25 mg/kg propofol can achieve sedation in neonatal rats, while 75 mg/kg can achieve a satisfactory anesthesia state for surgery without causing abnormal internal environment. In group S, the concentration of propofol in the thalamus was significantly higher than that in other brain regions (P<0.05), and there was no significant difference among other regions. In group A, the concentration of propofol in frontal and parietal cortex was significantly lower than that in other brain regions, and the concentration of propofol in hippocampus and cingulate gyrus was significantly higher than that in other brain regions (P<0.05), and there was no significant difference in other brain regions.

　　Conclusion: Intraperitoneal injection of propofol is a suitable anesthesia method for neonatal rats, and the distribution of propofol in different brain regions is different when the dose of propofol is different.