动物造模 z-bio 2022-06-14 315

　　Objective: To investigate the effect of ambient temperature on body weight, brown adipose tissue (BAT) thermogenesis and uncoupling protein 1 content of Chinese tree shrew, and to provide a theoretical basis for establishing a tree shrew obesity model.

　　Methods: 40 adult shrews with similar body weight were randomly divided into 5 groups (8 in each group): control group (0 d), kept at (25±1) ℃, 12 L/12 D; And placed at (5 ± 1) ℃, 12 L/12 D conditions were acclimated for 7, 14, 21 d and 28 d groups. After acclimation, the body weight, non-shivering thermogenesis (NST), brown adipose tissue weight and the content of uncoupling catenin 1 (UCP1) were determined.

　　RESULTS: Compared with the control group (0 d), the body weight, NST, BAT weight and UCP1 content of Chinese shrew in the cold acclimation group were significantly increased, and the color of BAT was also significantly deepened. After 28 d of cold acclimation, the body weight increased by 26.32%. , NST increased by 20.65%, BAT weight increased by 53.85%, and UCP1 content increased by 43%. UCP1 content was significantly positively correlated with BAT weight and NST.

　　Conclusion: The shrew may induce BAT tissue hyperplasia and up-regulation of UCP1 expression through cold acclimation, thereby enhancing the thermogenic activity of BAT to increase energy expenditure. It is speculated that BAT may be a target organ for the treatment of obesity by energetics.