动物造模 z-bio 2022-06-16 405

　　Objective To detect the effect of Jiayankangtai granules on immune-related cytokines in autoimmune thyroiditis rats, and to explore the mechanism of Jiayankangtai granules in improving autoimmune thyroiditis.

　　Methods Thirty female Lewis rats were randomly selected as normal group, 10 rats, and the remaining 20 rats were injected subcutaneously with thyroglobulin combined with oral iodine for 7 weeks. Rats with successful modeling were randomly divided into 10 model group and 10 administration group. After continuous intervention of Jiayankangtai granules for 8 weeks, the level of thyroid antibodies was detected by ELISA; the pathological changes of thyroid were observed by HE staining; the gene expression of target factors was detected by qPCR; the expression of target proteins was detected by antibody chip method and a cluster heat map was drawn.

　　Results TPOAb and TGAb in the model group were significantly higher than those in the normal group, and TPOAb and TGAb in the administration group were significantly lower than those in the model group. The thyroid follicular cells were destroyed and a large number of lymphocytes infiltrated in the model group, and the pathological changes of the thyroid in the administration group were relieved compared with the model group. The gene expression levels of IFN-γ, TNF-α, IL-17, CCL3, and CX3CL1 in the model group were higher than those in the normal group, and the levels of IL-4, IFN-γ, TNF-α, IL-17, CCL3, and CX3CL1 in the treatment group were higher than those in the model group. group decreased. The cytokines clustered well among the groups, the protein expression of IL-17, CCL3 and CX3CL1 in the model group was higher than that in the normal group, and the levels of IL-4, IFN-γ, TNF-α, CCL3 and CX3CL1 in the treatment group were lower than those in the model group. .

　　Conclusion Jiayankangtai granules can regulate immune-related cytokines and reduce thyroid injury in autoimmune thyroiditis rats.