动物造模 z-bio 2022-06-16 406

　　Objective To investigate the effect of membranous cells on chemotherapy-injured granulosa cells and the intervention effect of Zuogui Pill-containing serum.

　　Methods The drug-containing serum of Zuogui Pills was prepared, and the rat ovarian granulosa cells and theca cells were cultured. They were divided into 8 groups: (1) blank control group; (2) co-culture group; (3) ZGW serum group; (4) ZGW Serum + co-culture group; (5) model group; (6) model + co-culture group; (7) model + ZGW serum group; (8) model + ZGW serum + co-culture group. The expressions of Bax, Caspase-3, Beclin-1, p62 and LC3B at the transcriptional and translational levels were detected by real-time fluorescence quantitative PCR (qRT-PCR) and Western blot, respectively.

　　Results Beclin-1, LC3B, Bax and Caspase-3 genes in the model group were highly expressed at the transcriptional and translational levels, and the addition of 10% Zuogui Pill-containing serum or co-culture with membranous cells could down-regulate the expression of these genes The expression of p62 gene was lower than that of the control group, and the addition of 10% Zuogui Pill-containing serum or co-culture with membranous cells could up-regulate the expression of p62 gene in the model group. When the experimental group and membranous cells were co-cultured with 10% Zuogui Pill-containing serum, the changes in the expression of five genes were more obvious.

　　Conclusion The mesangial cells can alleviate the chemotherapeutic injury of granulosa cells in vitro, and have a synergistic effect with the drug-containing serum of Zuogui Pills, which is related to the inhibition of autophagy and the reduction of apoptosis of granulosa cells.