动物造模 z-bio 2022-06-16 370

　　Objective To observe the effects of Ginkgo Mihuan oral solution (referred to as Ginkgo biloba) on the proliferation, migration, cell tubule formation and protein expression of VEGF (vascular endothelial growth factor) and CD31 (platelet-endothelial cell adhesion molecule) in CMECs (rat myocardial microvascular endothelial cells). To explore the angiogenesis ability of silver honey in vitro.

　　Methods CMECs were cultured and divided into control group, Yinmi high (2.6mg/mL), medium (1.3mg/mL) and low (0.6mg/mL) dose groups. The proliferation ability of CMECs was detected by CCK-8 method; the migration ability of CMECs was detected by cell scratch assay; the tube-forming ability of CMECs was detected by Matrigel angiogenesis assay; the protein expressions of VEGF and CD31 were detected by Western blot.

　　Results Compared with the control group, the high, medium and low doses of Yinmi could significantly promote the proliferation of CMECs; and the cell proliferation rate in the high group was significantly higher than that in the medium and low groups. 6h after scratching, the cell migration rate of Yinmi high group was significantly higher than that of control group, and the cell migration rate of Yinmi medium and low groups was higher than that of control group, but there was no statistical difference; 24h after scratching, CMECs migrated in Yinmi high, medium and low groups The cell migration rate of Yinmi high group was higher than that of Yinmi medium and low groups. The number of meshes, mesh area, and number of intersections of CMECs in the Yinmi high group were significantly better than those in the control group, and the Yinmi medium and low groups. The expressions of VEGF and CD31 proteins in Yinmi high and middle groups were significantly increased.

　　Conclusion Ginkgo biloba oral solution can promote the proliferation, migration and angiogenesis of CMECs in vitro, and at the same time promote the expression of VEGF and CD31 proteins, and the efficacy is enhanced with the increase of the dose of Ginkgo biloba, suggesting that the oral solution of Ginkgo biloba has a significant effect on promoting in vitro vascularization. Angiogenesis capacity.