动物造模,肝纤维化 z-bio 2022-06-16 383

　　Objective To investigate the effect of IκB-α and its phosphorylation to down-regulate the nuclear transcription factor signaling pathway to reduce carbon tetrachloride-induced liver fibrosis in C. chinensis.

　　Methods The CCl4 liver fibrosis rat model was used to detect serum alanine aminotransferase (ALT) and hydroxyproline (HYP) biochemically, and ELISA to detect transforming growth factor β1 (TGF-β1) and α-smooth muscle actin (α-SMA). ) levels, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and fluorescence quantitative PCR to detect Toll-like receptor 4 (TLR4) mRNA, NF-κB p65 mRNA, NF-κB p65 mRNA in rat liver tissue The expression of κB inhibitory protein-α (IκB-α) mRNA, the protein expression of TLR4, NF-κB p65 and p-IκB-α in rat liver tissue were detected by Western blot, and the pathological damage of liver tissue was evaluated by HE staining.

　　RESULTS: Compared with the model group, Coriander L. can significantly inhibit the levels of ALT, HYP, TGF-β1, α-SMA, IL-6 and TNF-α in rats with liver fibrosis, and improve the pathological damage of liver tissue in rats. Significantly inhibited the expression of TLR4 mRNA and NF-κB p65 mRNA, up-regulated the expression of IκB-α mRNA, down-regulated the expression of TLR4 and NF-κB p65 protein, and down-regulated the expression of p-IκB-α protein in liver fibrosis rats.

　　Conclusion The three-leaf coriander can down-regulate the phosphorylation of IκB-α, inhibit the activation of NF-κB signaling pathway, and achieve the effect of anti-hepatic chronic inflammatory injury.