动物造模,肠道菌群 z-bio 2023-02-28 399

　　Objective To investigate the effects of two reoviruses, T1L and NBV, on the structure of intestinal flora in mice.

　　Methods Twenty-five mice were randomly divided into 5 groups (control group, NBV intranasal administration group, NBV intragastric administration group, T1L intranasal administration group, T1L intragastric administration group), 5 mice in each group. The control group was given PBS by gavage, and the other groups were infected with 2 × 107 PFU/mL virus titer. After 7 days, mouse feces were collected, and 3 samples with heavier feces were selected from the 5 samples in each group. The V3+V4 variable region-specific amplification of fecal DNA was performed, and 16S rRNA sequencing was used to analyze the feces of mice. The abundance, diversity and species composition of the flora.

　　Results The abundance and diversity of intestinal flora of mice after administration of T1L and NBV decreased compared with the control group, and the decrease was most significant in the T1L intranasal group (P<0.05); The abundance and diversity of flora in the NBV nasal drop group increased significantly (P<0.05). At the phylum level, the abundance of Firmicutes was significantly reduced in the T1L and NBV gavage groups, and the abundance of Bacteroidetes was significantly reduced in the T1L and NBV intranasal groups; at the genus level, T1L intranasal The abundance of Romboutsia in the T1L gavage group, T1L gavage group and NBV gavage group was significantly decreased, while the abundance of Alistipes in the T1L intranasal group was significantly increased (P<0.05).

　　Conclusion T1L and NBV infection of mice can reduce the abundance and diversity of bacterial flora, which may disrupt the balance of bacterial flora by reducing beneficial bacteria or increasing pathogenic bacteria. In addition, the infection mode of the virus is different, and the impact on the flora is also different.