动物造模 z-bio 2022-06-16 762

　　Objective To anesthetize C57BL/6J mice with different doses of sutai combined with xylazine hydrochloride, observe the anesthesia-related time of mice under different anesthesia doses, and provide reference for various types of mouse operations and related experimental design.

　　Methods 100 C57BL/6J mice (half male and female) were randomly divided into 5 groups with 20 mice in each group (half male and female). The mice in the 4 administration groups were treated according to 55 mg/kg of shutai + 13.75 mg/kg of xylazine hydrochloride, 65 mg/kg of shutai + 16.25 mg/kg of xylazine hydrochloride, 75 mg/kg of shutai + 18.75 mg of xylazine hydrochloride mg/kg, sutai 85 mg/kg + xylazine hydrochloride 21.25 mg/kg for intraperitoneal injection; after the mice's righting reflex disappeared, 1 drop (about 20 µL) of Xinrodun eye drops was instilled in each eye. , place the mice on the thermal pad. Mice in the control group were intraperitoneally injected with 200 µL of 0.9% sodium chloride solution. Observe, record and compare the anesthesia induction time, anesthesia maintenance time and anesthesia recovery time of each group of mice. Mice were reared for 1 d after recovery from anesthesia, and serum was collected. Hitachi 7080 automatic biochemical analyzer was used to determine liver and kidney function indexes: alanine aminotransferase, aspartate aminotransferase, and creatinine.

　　Results The success rate of sutai combined with xylazine hydrochloride to anesthetize C57BL/6J mice was 100%. When the anesthesia dose of sutai is between 55 and 75 mg/kg, it can quickly and safely induce anesthesia in mice, and the anesthesia effect is good. The maintenance time of anesthesia was proportional to the injection dose of Shutai (r2=0.827 in male rats, r2=0.841 in female rats, all P<0.05), and the induction time of anesthesia was inversely proportional to the injection dose (r2=0.432 in male rats, r2=0.410 in female rats). , P values were all < 0.05). After recovery from anesthesia, there was no significant difference in liver and kidney function indexes of mice in each group compared with the control group (P>0.05).

　　Conclusion Using 55-75 mg/kg sutai combined with xylazine hydrochloride to anesthetize C57BL/6J mice can obtain good anesthesia effect and is safe, and the anesthesia time can be controlled by adjusting the anesthetic dose, suggesting that this is a stable and reliable method. Anesthesia method.