动物造模 z-bio 2022-06-16 344

　　Objective To construct a dural defect model in New Zealand white rabbits, and to evaluate the effectiveness of the biodegradable patch in repairing dural defect by observing the physiological state, clinical manifestations and histopathological changes of the animals after using the domestic porcine small intestinal submucosa acellular matrix patch. and security.

　　Methods Forty-eight New Zealand white rabbits were used to make dural defect models, and they were randomly divided into experimental group and control group, with 24 rabbits in each group. The sold biological dura mater patch (control) was used to observe the basic conditions of the two groups of animals after surgery. The changes of hematology and blood biochemical indexes of the rabbits were detected at 7 d, 30 d, 60 d, and 90 d after implantation, respectively, and then the animals were euthanized, and the dura mater was dissected to observe the repair of the dura. Histological analysis. During the experiment, veterinary clinical observation was performed on all animals.

　　RESULTS: Both the experimental group and the control group could heal the epidural orifice 30 days after the operation. There was no obvious abnormality in the clinical behavioral observation of the two groups of animals, and there was no difference in the survival rate. In addition, there were no significant changes in rabbit hematology and biochemical indexes at each time point after implantation (P>0.05), and the observation after dissection of the animals confirmed that there was no significant difference in the repair process of the dura between the two groups, and there was no pathological examination. Infectious inflammatory response.

　　Conclusion The use of porcine small intestinal submucosa acellular matrix to repair rabbit dura mater has similar dura mater repair efficacy and anti-cerebrospinal fluid leakage effect compared with commercially available materials, which proves its safety and effectiveness.