动物造模,坏死性小肠结肠炎 z-bio 2022-12-17 678

　　Objective To analyze the protective effect of amygdalin on necrotizing enterocolitis (NEC) in neonatal rats.

　　Methods Sixty healthy newborn SD rats were randomly divided into control group, model group, low-dose amygdalin group, amygdalin medium-dose group, amygdalin high-dose group and sulfasalazine according to the random number table method. groups, 10 animals in each group. Except for the control group, rats in other groups were used to establish the NEC model by hypoxia-cold stress combined with formula milk invasive feeding method. At the same time, the low, medium and high dose groups were given 20, 40, and 80 mg/kg amygdalin respectively, and the sulfasalazine group was given 300 mg/kg sulfasalazine for 5 consecutive days. 12 h after the last intervention, the body weight changes of the rats were recorded, the small intestine ileocecal tissue was taken for HE staining, and the ileocecal intestinal tissue injury score was performed; the apoptosis rate was detected by TUNEL staining; serum inflammatory factors were detected by enzyme-linked immunosorbent assay. : Tumor necrosis factor (TNF-α), interleukin-6 (Ⅱ-6), interleukin-1β (Ⅱ-1β), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione Glypeptide peroxidase (GSH-Px) levels. The protein expression levels of NOD-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein (ASC) and caspase-1 were detected by Western blot.

　　Results Compared with the control group, the body weight of the rats in the model group decreased significantly. The expression levels of ASC and Caspase-1 protein were significantly increased (P<0.05); compared with the model group, the body weight of the rats in the chasenoside medium-dose group, the sulfasalazine high-dose group, and the sulfasalazine group increased significantly. Intestinal tissue injury score, the apoptosis rate of intestinal tissue cells was significantly decreased, the levels of TNF-α, I-6, IL-1β, SOD, MDA, GSH-Px, NLRP3, ASC, and Caspase-1 protein expressions were significantly decreased (P< 0.05).

　　Conclusion Amygdalin has a certain protective effect on necrotizing enterocolitis in neonatal rats, which can effectively reduce intestinal tissue damage, reduce the body's inflammatory response, oxidative stress response, and reduce the apoptosis rate of intestinal tissue cells, but the specific response mechanism is not enough. It is clear that further clinical investigation is needed.