动物造模,溃疡性结肠炎 z-bio 2023-03-01 476

　　Objective To observe the effect of mussel mucin (MAP) on the intestinal mucosa of rats with ulcerative colitis, and to explore its mechanism from the perspective of adhesion and anti-oxidation.

　　Methods Thirty-two SPF SD rats were randomly divided into normal group, model group, mesalazine group, and mussel mucin group (0.6 mg/kg), with 8 rats in each group. The normal group was given ordinary drinking water, and the other groups were given 5% dextran sodium sulfate (DSS) to drink water freely for 7 days to prepare the ulcerative colitis model. The rats in each group were given daily administration for 24 hours after the establishment of the model. The daily disease activity index (DAI) and body weight changes were measured. After 7 days of administration, the rats were dissected and collected, and the colorectal length, weight, and intestinal weight ratio of the rats in each group were recorded. The rat intestinal mucosa was used for in vitro test, and the effect of MAP on the adhesion and antioxidant effect of rat intestinal mucosa was observed by nitrotetrazolium chloride (NBT) staining method.

　　Results Compared with the normal group, the DAI score of the model group increased significantly from the 5th day of the experiment (P<0.05), the body weight decreased (P<0.05), and the intestinal mucosal congestion, edema and ulceration, and colon The crypts were swollen and deformed, and a large number of inflammatory cells infiltrated in the submucosal tissue; compared with the model group, the body weight and colorectal length of the mesalazine group and the mussel mucin group increased (P<0.05), DAI score, intestinal Gravity ratio, CMDI score and histopathological score were all decreased (P<0.05). In the NBT experiment, it can be seen that the rectal mucosa of rats appears blue-stained, and the degree of blue-staining is further deepened with time.

　　Conclusion MAP can significantly improve the symptoms of ulcerative colitis model rats and repair the damage of intestinal mucosal barrier through good adhesion to intestinal mucosa and antioxidant effect, which can provide a reliable experimental basis for the treatment of ulcerative colitis by MAP.