动物造模,急性肺损伤 z-bio 2023-02-27 597

　　Objective To investigate the protective effect of etomidate (Eto) on acute lung injury in rats with septic shock based on the Toll-like receptor 4 (TLR4) pathway.

　　Methods Seventy-five rats were randomly divided into sham operation group, model group, model-lipopolysaccharide (LPS) group, model-Eto group, and model-Eto-LPS group, with 15 rats in each group. Except for the sham-operated group, the other groups were treated with cecal ligation and puncture to replicate the septic shock rat model, and the sham-operated group only underwent laparotomy. Thirty minutes before surgery, the model-LPS group was intraperitoneally injected with LPS 15 mg/kg, the model-Eto group was intraperitoneally injected with etomidate 60 mg/kg, and the model-Eto-LPS group was also intraperitoneally injected with etomidate 60 mg/kg+LPS 15 mg/kg, the model group and the sham-operated group were intraperitoneally injected with normal saline. The levels of serum endotoxin (ET), IL-1β, IL-6 and TNF-α in BALF were detected 5 h after operation, the pathological changes of lung tissue were observed, and TLR4, myeloid differentiation factor (MyD88), nuclear factor in lung tissue were detected. -κB p65 (NF-κB p65) mRNA and protein expression.

　　Results Compared with the sham operation group, the model group, model-LPS group, model-Eto group, model-Eto-LPS group had higher levels of ET and IL-1β, IL-6 and TNF-α in BALF, among which model- Eto group

　　Conclusion Eto has a protective effect on acute lung injury in septic shock rats, which may play a regulatory role by inhibiting the TLR4 pathway.