动物造模,特应性皮炎模型 z-bio 2022-06-17 277

　　Objective To investigate the efficacy and preliminary mechanism of oxymatrine, the active ingredient in Sophora flavescens, in the treatment of atopic dermatitis mice.

　　Methods (1) KM mice were randomly divided into 5 groups: blank control group, model control group, positive control group and oxymatrine low- and high-dose (50, 100 mg/kg) groups, administered twice a day, After 7 consecutive administrations, 10 min after the last administration, 50 μL of dextran was subcutaneously injected into the left hind paw pad of the mice, and the number of licking of the hind paws of the mice within 15 min was observed and recorded. (2) BALB/c mice were randomly divided into 6 groups: blank control group, model control group, positive control group and oxymatrine low, medium and high dose (25, 50, 100 mg/kg) groups. Except for the blank control group, the other 5 groups of mice were all stimulated with 2,4-dinitrochlorobenzene (DNCB) repeatedly to establish AD models. From the first day of the experiment, each group was given corresponding vehicles or drugs by gavage, and the intervention was continued. 14d. The body weight changes of AD model mice were observed and recorded, the organ coefficients of spleen and thymus were determined, the contents of total IgE and Th2 cytokines in peripheral blood were determined by ELISA, the histopathological changes of skin lesions were observed by HE staining, toluidine blue The infiltration of mast cells in the skin lesions was observed by staining.

　　Results Oxymatrine could significantly inhibit the scratching frequency of pruritus model mice (P<0.01). After 14 days of oxymatrine treatment by gavage, AD model mice eczema-like dermatitis was relieved: pathological section results showed that mice The thickening of the dermis was reduced, the number of inflammatory cells and intercellular edema were reduced; the number of mast cells infiltrated in the dermis was significantly reduced, and the expressions of total IgE and Th-type cytokines IL-4 and IL-13 in peripheral blood were significantly reduced ( P<0.05).

　　Conclusion Oxymatrine has a certain therapeutic effect on AD model mice, and its mechanism is related to inhibiting the infiltration of mast cells in the dermis of the skin and reducing the expression of Th2 cytokines.