动物造模,糖尿病足溃疡 z-bio 2022-06-20 292

　　Objective To investigate the effect of salidroside (Sal) on nuclear transcription factor E2-related factor 2 / Kelch-like epichlorohydrin-related protein 1 (Nrf2 / Keap1) signaling pathway and wound healing in diabetic foot ulcer (DFU) rats.

　　Methods The diabetic rat model was established by feeding with high-fat and high-sugar diet combined with intraperitoneal injection of streptozotocin (STZ), and the back of the foot was depilated and cut to the fascia, and an ulcer wound with an area of about 3 mm × 7 mm was created. DFU rat model was randomly divided into DFU model group (DFU group), low Sal (Sal-L, 0.1 g / (kg·d)), medium (Sal-M, 0.2 g / ( kg·d) )) high (Sal-H, 0.3 g / (kg·d)) dose group, positive drug metformin group (MET group, 0.65 g / (kg·d)), and normal blood sugar wound rats were set as The control group (NC group) was given continuous gavage for 2 weeks. The body weight and fasting blood glucose (FBG) level of the rats in each group were detected on the 7th and 14th days of treatment, the expression of CD34 in the wound tissue was detected by immunohistochemistry, and the microvessel density (MVD) of the wound surface was calculated; SOD level; Western blot was used to detect the expression of Nrf2 and Keap1 proteins in wound tissue.

　　Results On the 0th day of treatment, there was no significant difference in the body weight of the rats in each group (P>0.05). The DFU group, the Sal-L group, the Sal-M group, the Sal-H group and the MET group had no significant difference in FBG in each group. The levels were higher than those in the NC group (P<0.05); on the 7th and 14th days of treatment, compared with the NC group, the rats in the DFU group, the Sal-L group, the Sal-M group, the Sal-H group and the MET group Body weight, FBG level, MDA content and Keap1 protein expression were all increased (P<0.05), while wound healing rate, CD34 positive cells, MVD, SOD activity and Nrf2 protein expression were significantly decreased (P<0.05); Compared with the DFU group, the body weight, FBG level, MDA content and Keap1 protein expression of the rats in the Sal-L group, the Sal-M group, the Sal-H group and the MET group were significantly decreased (P<0.05). , CD34 positive cells, MVD, SOD activity and Nrf2 protein expression were significantly increased (P<0. sal-l="" met="" p="">0.05).

　　Conclusion Sal may increase the antioxidant capacity of DFU rats and promote wound healing by regulating the Nrf2/Keap1 signaling pathway.