动物造模 z-bio 2022-06-22 279

　　Objective To investigate the protective effect of curcumin on acute kidney injury induced by gestational hypertension in rats and explore its potential mechanism.

　　Methods Thirty-two pregnant Sprague-Dawley female rats were divided into four groups: normal group, curcumin group, pregnancy induced hypertension (PIH) group and PIH+curcumin group. Rats in PIH group and PIH+curcumin group were injected with 26 mol/L NG-nitro-L-arginine methyl ester every day to induce PIH model for 4 days. Rats in the curcumin group and PIH+curcumin group were fed curcumin 204.8 mol/L (suspended in 2% carboxymethyl cellulose solution) from day 18 for 7 days. A series of indicators were detected by hematoxylin-eosin staining, enzyme-linked immunosorbent assay and spectrophotometry, respectively, to evaluate the pathological morphology, renal function, oxidative stress and inflammatory response of renal tissue. The expression and distribution of TGF-β1 and p-Smad3 were detected by immunohistochemistry and western blotting.

　　Results Compared with the PIH group, the renal function of the PIH+curcumin group was significantly improved (P<0.01), and the renal tissue damage and oxidative stress were also significantly recovered (P<0.01). (191. 23 ± 18. 82) decreased to (108. 35 ± 11. 24) (P < 0. 01). The levels of TGF-β1 and p-Smad3 in the kidneys of rats in the PIH group were significantly increased, but were significantly decreased after the application of curcumin. )% (P<0.01), while p-Smad3/Smad3 decreased from (312.82±30.26)% to (143.05±15.31)% (P<0.01).

　　Conclusion Curcumin can protect acute kidney injury in pregnant hypertensive rats by regulating TGF-β1 pathway.