动物造模,巨细胞病毒 z-bio 2023-03-16 464

　　Objective To study the inhibitory effect of cytomegalovirus on the immune response of mice implanted with polymethyl methacrylate.

　　Methods The CMV infection mouse model was established by intraperitoneal inoculation of virus suspension. Seven days after virus inoculation, PMMA was implanted into the back of mice subcutaneously; flow cytometry was used to detect the proportion of monocytes/macrophages, CD4+ T cells, CD8+ T cells and regulatory T cells (Treg cells) in peripheral blood. The content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) was detected by enzyme-linked immunosorbent assay (ELISA).

　　Results Compared with the implanted control group, the proportion of MI type monocytes/macrophages in the peripheral blood of the mice implanted with CMV decreased by 73.18% (=5.896, P=0.004), and the ratio of M2 type monocytes/macrophages It increased by 2.18 times (t=7.971, P=0.001), the ratio of CD4/CD8 T cells decreased by 42.91% (t=6.468, P=0.003), and the proportion of Treg cells increased by 2.49 times (t=4.495, P =0.011). Compared with the implanted control group, the concentrations of TNF-α and IL-1β in the peripheral blood of the CMV-implanted mice were decreased by 71.65% and 57.95%, respectively (t=7.236, P=0.019, t=7.543, P=0.002)

　　Conclusion CMV can significantly inhibit the immune response after PPMA implantation, which provides a theoretical basis for the antiviral treatment of CMV-infected patients after oral biomaterial implantation.