动物造模 z-bio 2022-06-22 296

　　Objective To investigate the effect of α-mangostin on the immune function of cyclophosphamide-induced immunosuppression in mice, and to provide experimental basis for the follow-up mechanism research and the development of new drugs to increase immune function.

　　Methods Mice were randomly divided into 5 groups with 10 mice in each group: blank control group, immunosuppression group, α-mangostin high-dose group, middle-dose group and low-dose group. In the first 7 days, except the blank control group, the other 4 groups were given cyclophosphamide (CTX) to establish the immunocompromised model; after 14 days, the blank control group continued to be given normal saline, and the immunosuppressed group was given The three dose groups were given different doses of α-mangostin. Mice were sacrificed 24 h after the last administration, and the delayed-type allergy (DTH) detection, serum hemolysin (HC50), macrophage phagocytic function (carbon clearance test), and immune organ thymus and spleen index were measured, and the natural Killer cell (NK cell) activity assay (MTT method), determination of peripheral blood white blood cell count, and spleen lymphocyte proliferation assay to study the effect of α-mangostin on the immune function of mice.

　　Results The mouse model of immunosuppression was successfully created. After gavage of α-mangostin, the three dose groups could increase the thymus and spleen index, half hemolysis value, spleen lymphocyte proliferation rate and NK cell activity in immunosuppressed mice to different degrees, among which the high-dose group, the total α-mangostin The intragastric dose of 100 mg/(kg·d) had the most prominent effect. In multiple experiments, compared with the immunosuppressed group, the difference was extremely significant and statistically significant (P<0.01); In delayed-type allergy, except the high-dose group was significantly different from the blank control group and the immunosuppression group (P<0. p="">0. 05); Compared with the immunosuppressed group, there was a very significant difference (P<0. p="">0.05); in the carbon clearance experiment, there was no statistical significance between each dose group compared with the blank control group and the immunosuppressed group (P> 0.05).

　　Conclusion Mangostin has a regulatory effect on the immune function of immunosuppressed mice, and the optimal dose for improving the immune function of immunosuppressed mice is more inclined to the high-dose group, which is dose-dependent within a certain range.