动物造模 z-bio 2022-06-22 252

　　Objective To observe and analyze the effects of lipopolysaccharide on the morphological structure and pathological indexes of the lungs of naked mole-rats, and to preliminarily explore whether the naked mole-rats have LPS tolerance.

　　Methods Ten male naked mole rats were randomly divided into experimental group and control group. The experimental group was intraperitoneally injected with LPS at a dose of 10 mg/kg according to the body weight, and the control group was injected with the same volume of 0.9% NaCI solution (physiological saline). 6h after administration, lungs were collected, and the wet-dry weight ratio of lung tissue was detected; paraffin was made

　　Sections were stained with HE, and the microscopic structure of lung tissue was observed under an optical microscope; the content of reactive oxygen species in lung tissue cells and the apoptosis rate of lung tissue cells were detected by flow cytometry.

　　Results The lung tissue structure of naked mole rats in the experimental group was intact, and there were no obvious lesions such as exudate and inflammatory cell infiltration in the alveolar pores. Compared with the control group, the wet-dry weight ratio of lung tissue in the experimental group did not change significantly, the early apoptosis rate did not change significantly, but the late apoptosis rate was significantly increased (P<0.01), and the reactive oxygen species content was significantly increased (P<0.01 ). 0.05).

　　Conclusions Naked mole rats have a certain degree of tolerance to LPS-induced lung injury, and the specific mechanism needs to be further studied.