动物造模 z-bio 2023-03-16 441

　　Objective To study the changes of tumor necrosis factor-α, interleukin-1β and C-reactive protein in the process of inflammatory reaction in the Phenma pig model of plateau multiple organ dysfunction syndrome.

　　Methods Three doses (0.25, 0.35 and 0.50 mg/kg) of endotoxin lipopolysaccharide (LPS) were used to induce the H-MODS model (groups B, C, and D) of plateau native pigs. control group (group A). The expression levels of serum TNF-α, IL-1β and CRP at different time points were detected.

　　Results The expression levels of TNF-α and IL-1β increased sharply in each group after LPS was pumped into the pigs, and reached the highest at 6 h. The ratio of TNF-α increased significantly (P≤0.05 or P≤0.01), and gradually decreased after 12 h; TNF-α expression in groups B and D increased significantly after 24 h compared with 0 h in the same group and group A at the same time point. High (P<0.05), group B was significantly higher than group A (P<0.05) after 72 h. The expression level of IL-1β at 24 h in group D was higher than that in groups 0 h and A (P<0.05), and then gradually decreased. <0.05 or P<0.01). After intravenous infusion of LPS, the expression of CRP in groups B, C, and D began to increase, and reached the maximum at 24-48 h; The ratios were significantly increased (P<0.05 or P<0.01).

　　Conclusion When H-MODS is formed, the body produces a severe inflammatory response, and the expressions of inflammatory factors TNF-α and IL-1β increase rapidly, but their expressions are rapidly down-regulated and fluctuate sharply in a short period of time under the action of immune regulation. The equilibrium state is reached; while the change of CRP expression is relatively slow, which is accompanied by the whole process of inflammatory response in vivo.