动物造模 z-bio 2022-11-29 413

　　Objective To investigate the effect of resveratrol (RSV) on the lymphotoxin analog (LIGHT)/herpesvirus intervening body (HVEM) pathway in preeclampsia (PE) rats, and its protective effect on renal injury.

　　Methods Fifty SD pregnant mice were randomly divided into normal pregnant mice group (Normal group), preeclampsia model group (PE group), resveratrol (RSV) low (50 mg/kg), high (200 mg/kg) ) dose group, LIGHT/HVEM pathway blocker (lymphotoxin beta receptor, LTβR-Ig fusion protein, 1600 μg/kg, LTβR-Ig group), 10 rats in each group. Except for the Normal group, rats in other groups were intraperitoneally injected with nitroso-L-arginine methyl ester (L-NAME, 300 mg/kg, for 5 days, once a day) to establish a rat model of preeclampsia, and the modeling was successful. After starting administration, RSV groups were given corresponding doses of RSV solution by gavage, LTβR-Ig group was given LTβR-Ig solution via tail vein injection, Normal group and PE group were given the same amount of normal saline by gastric gavage + tail vein injection, and each group was given continuous Administration for 5 days, once a day. After the last administration, 24 h urine was collected from the rats, and blood was taken, and enzyme-linked immunosorbent (ELISA) kits were used to detect renal function indexes serum creatinine (Scr), blood urea nitrogen (BUN) and 24 h urinary protein in urine. ; Hematoxylin-eosin (HE) staining was used to detect the pathological changes of renal tissue in each group; real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the relative levels of LIGHT and HVEM mRNA in renal tissue; Western blotting (Western blotting) The relative expression levels of LIGHT, HVEM, nuclear transcription factor-κB (NF-κB), and interleukin-6 (IL-6) in renal tissue were detected by blot).

　　Results Compared with the Normal group, the rats in the PE group had pathological damages such as diffuse proliferation of glomerular endothelial cells, serum Scr, BUN and 24-h urinary protein in urine, LIGHT mRNA and protein, HVEM mRNA and protein, and mRNA and protein in kidney tissue. The protein expressions of NF-κB and IL-6 were increased (P<0.05); compared with the PE group, the renal tissue pathological damage in the RSV low-dose, high-dose and LTβR-Ig groups was relieved, and serum Scr, BUN and urine were significantly higher than those in the PE group. The 24-hour urine protein level, LIGHT mRNA and protein, HVEM mRNA and protein, NF-κB and IL-6 protein in kidney tissue were decreased (P<0. rsv="" r-ig="" p="">0 .05).

　　Conclusion RSV can inhibit the expression of LIGHT/HVEM pathway protein in renal tissue of PE rats, and improve PE kidney injury.