动物造模 z-bio 2022-10-13 377

　　Objective To investigate the effect of agmatine on propofol-induced neurotoxicity in neonatal rats.

　　Methods The rats were divided into 5 groups by intraperitoneal injection of propofol to induce nerve injury model: Control group, Propofol group, Propofol + Agmatine 1. 25 mg/kg group, Propofol + Agmatine 2. 5 mg/kg group and Propofol + Agmatine group 5 mg/kg group. The number of mistakes was detected by the platform jump test, the brain water content and brain index were detected by the 2,3,5-triphenyltetrazolium chloride method, the neurological deficit score was evaluated by the Longa method, and the postural reflex score was detected by HE staining to detect the degree of pathological damage in the hippocampus. Nissl Apoptosis in hippocampus was detected by staining, and protein expression levels of BDNF, NGF, Bax, Bcl-2, Nrf2, p-Nrf2 and HO-1 were detected in brain tissue of rats in each group by western blot.

　　Results The results showed that compared with the Control group, the Propofol group significantly increased the number of mistakes (P<0.05), the brain water content and the brain index (P<0.05), the neurological deficit score and the postural reflex score were significantly increased (P<0.05). P<0.05), showed obvious pathological damage, the protein levels of BDNF and NGF were significantly decreased (P<0.05), the number of Nissl bodies was significantly decreased, the ratio of Bax/Bcl-2 was significantly increased (P<0.05), p-Nrf2/Nrf2 The ratio and HO-1 protein level were significantly decreased (P<0.05). Compared with the Propofol group, the Propofol+Agmatine 2. 5 and 5 mg/kg groups significantly reduced the number of mistakes (P < 0. 05), the brain water content, the brain index (P < 0. 05), and the neurological deficit scores. , Postural reflex scores were significantly decreased (P<0.05), the degree of pathological damage was significantly improved, BDNF and NGF protein levels were significantly increased (P<0.05), the number of Nissl bodies was significantly increased, and the Bax/Bcl-2 ratio was significantly decreased (P<0.05). P<0.05), p-Nrf2/Nrf2 ratio and HO-1 protein level were significantly increased (P<0.05).

　　Conclusion Agmatine alleviates propofol-induced neurotoxicity in neonatal rats by regulating the Nrf2/HO-1 signaling pathway.