动物造模,肝纤维化 z-bio 2022-12-28 453

　　Objective To explore the characteristics and significance of intrahepatic shear wave velocity changes in mice with carbon tetrachloride (CCl4)-induced hepatic fibrosis using acoustic radiation force pulse elastography.

　　Methods 6-week-old male C57BL/6J mice were divided into 3 groups: the control group was given edible oil (0.1mL/20g body weight) by gavage once every other day for 8 weeks; the 4-week group was given the above-mentioned edible oil every other day for 1-4 weeks. Gavage once, the fifth week of the experiment, 1% CCl4 edible oil mixture (250 μL/kg) was administered by gavage, once every other day for 4 weeks; the 8-week group began to gavage CCl4 edible oil mixture (250 μL/kg) in the first week of the experiment. kg), once every other day for 8 weeks. After 8 weeks of experiment and 2 weeks of normal feeding after drug withdrawal, ARFI technology was used to detect intrahepatic shear wave velocity, then all mice were sacrificed, liver tissues were collected, HE and Sirius red collagen fibers were stained, and the pathological changes of liver tissue were observed.

　　Results Compared with the control group, the shear wave velocity in the liver increased after 2 weeks of normal feeding in the 4-week group, and the formation of fibrosis was observed in histological observation; while the shear wave velocity in the liver was higher in the 8-week group after 2 weeks of normal feeding, and the tissue Scientific observation showed the formation of liver cirrhosis. However, the 4-week group and the 8-week group had similar intrahepatic shear wave velocities at the end of exposure, which were significantly higher than those after 2 weeks of normal feeding.

　　Conclusion The hepatic shear wave velocity detected by ARFI technology can reflect the changes of liver fibrosis in mice, but the influence of intrahepatic inflammatory injury on the intrahepatic shear wave velocity must be excluded.