动物造模 z-bio 2022-06-23 269

　　Objective To observe the effect of ozone autoblood method on melatonin and oxidative stress in sleep deprived rats.

　　Methods Forty adult male Wistar rats were selected, and a rapid eye movement sleep (REM) sleep deprivation model was established by the "modified multi-platform sleep deprivation method". According to the principle of random number table, the rats were divided into control group and observation group, with 20 rats in each group. The rats in the observation group were given ozone autologous blood transfusion, and the rats in the control group were treated with air. At 0h, 24h, 48h and 72h of intervention, tail vein blood was collected to detect serum melatonin levels, and at 0h and 72h of intervention, venous blood oxidative stress indicators superoxide dismutase (SOD) and glutathione were detected. Peroxidase (GSH-px) and malondialdehyde (MDA) levels.

　　Results At 0h of intervention, there were no significant differences in wake (wake, W), slow-wave sleep (SWS), and fast-wave sleep (FWS) between the two groups of rats (P >0.05); at 72 h of intervention, the W of the observation group was lower than that of the control group, and the SWS and FWS of the rats were higher than those of the control group (P<0.05). At 48h and 72h of intervention, the serum melatonin level of the observation group was higher than that of the control group (P<0.05). After 72 hours of intervention, the levels of SOD and MDA in the observation group were lower than those in the control group, and the level of GSH-px was higher than that in the control group (P<0.05).

　　Conclusion Ozone auto-blood method can promote the release of melatonin in sleep-deprived rats, reduce the levels of serum SOD and MDA in rats, and increase the level of serum GSH-px in rats.