动物造模 z-bio 2022-06-23 239

　　Objective To study the toxicity of oral administration of different extracts of Psoralea in rats, and to provide a safe basis for its clinical application.

　　Methods A total of 112 SD rats were divided into 8 groups according to gender and body weight: control group, Psoralea crude drug group, Psoralea lower layer liquid high and low dose groups, Psoraleae upper layer Liao liquid group The high and low dose groups, the high and low dose groups of psoralea infiltration of medicinal residues [the crude drug group dose (crude drug amount) was 3 g/kg body weight, and the other high and low doses (crude drug amount) were 6 g/kg body weight, respectively. and 3g/kg body weight], 14 animals in each group. After continuous administration for 4 weeks, after the end of the administration period, fasting and drinking water for 12 hours, blood was collected for the detection of relevant blood biochemical indicators, the organs were dissected and weighed to calculate the organ coefficient and MDA in liver tissue was determined, and histopathological examination was performed.

　　Results Compared with the control group, the liver organ coefficient, ALP and MDA in the Psoralea crude drug group were significantly increased, while the thymus organ coefficient and T-AOC were significantly decreased. -AOC and ALT were significantly increased; the liver organ coefficient and MDA were significantly increased in the high-dose Psoraleat Radix Psoriasis group, while the T-AOC value of female rats was significantly decreased, and the low-dose drug residue group had no obvious liver and kidney Toxicity; there was no significant difference between the psoralen upper layer exudate group.

　　Conclusion The safety of the upper layer of psoraleae liquid is better than that of the lower layer of psoraleae, but based on the results of the determination of effective components, it is suggested that the lower layer of liquid is more suitable for human medicine.