动物造模,肠道菌群 z-bio 2022-06-23 399

　　Objective To investigate the effect of the microflora-sensing signal molecule N-acylhomoserine lactone on the composition of intestinal microflora in mice, so as to provide theoretical basis for the disturbance of intestinal microecology under infection.

　　Methods 3-oxo-C10-HSL molecules were injected into mice by intraperitoneal injection, and the duodenum and jejunum of mice were sampled by swab method at 24 hours, and the samples were analyzed by Illumina Hiseq 2500 high-throughput sequencing platform for 16S rDNA sequencing, and microbial flora taxonomic composition and diversity analysis.

　　Results A total of 22 bacterial phyla and 109 bacterial genera were detected in the intestinal tract of the experimental mice. ) and Proteobacteria (>20%), but there were significant differences in the genus-level flora composition between the two groups. The dominant genera in the control group were Staphylococcus, Escherichia-Shigella, Lactococcus, and Pseudomonas. Enterococcus, Enterococcus, the dominant bacteria in the 3-oxo-C10-HSL treatment group were mainly Enterococcus, Escherichia-Shigella, Staphylococcus: diversity and abundance analysis indicated that the 3-oxo-C10-HSL treatment group intestinal The diversity of tract flora increased, and the abundance of main dominant bacteria such as Escherichia coli increased significantly, and the colony abundance in the jejunum and ileum was the highest.

　　Conclusion AHLs have little effect on the composition of intestinal microflora in mice, but have more obvious effects on the structural changes of some specific groups within the microbiota.